Nectar yeasts in the tall Larkspur Delphinium barbeyi (Ranunculaceae) and effects on components of pollinator foraging behavior

PLoS One. 2014 Oct 1;9(10):e108214. doi: 10.1371/journal.pone.0108214. eCollection 2014.

Abstract

Microorganisms frequently colonize the nectar of angiosperm species. Though capable of altering a suite of traits important for pollinator attraction, few studies exist that test the degree to which they mediate pollinator foraging behavior. The objective of our study was to fill this gap by assessing the abundance and diversity of yeasts associated with the perennial larkspur Delphinium barbeyi (Ranunculaceae) and testing whether their presence affected components of pollinator foraging behavior. Yeasts frequently colonized D. barbeyi nectar, populating 54-77% of flowers examined depending on site. Though common, the yeast community was species-poor, represented by a single species, Metschnikowia reukaufii. Female-phase flowers of D. barbeyi were more likely to have higher densities of yeasts in comparison to male-phase flowers. Pollinators were likely vectors of yeasts, as virgin (unvisited) flowers rarely contained yeasts compared to flowers open to pollinator visitation, which were frequently colonized. Finally, pollinators responded positively to the presence of yeasts. Bombus foragers both visited and probed more flowers inoculated with yeasts in comparison to uninoculated controls. Taken together, our results suggest that variation in the occurrence and density of nectar-inhabiting yeasts have the potential to alter components of pollinator foraging behavior linked to pollen transfer and plant fitness.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Behavior, Animal*
  • Colony Count, Microbial
  • Plant Nectar*
  • Pollination*
  • Ranunculaceae / microbiology*
  • Yeasts* / classification
  • Yeasts* / physiology

Substances

  • Plant Nectar

Grants and funding

This work was supported by the Dartmouth College Cramer Fund, the Jean H. Langenheim graduate fellowship from the RMBL, and the National Science Foundation (DEB-0841862 and DEB-1311156). Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.