Multiplex PCR for detection of seven virulence factors and K1/K2 capsular serotypes of Klebsiella pneumoniae

J Clin Microbiol. 2014 Dec;52(12):4377-80. doi: 10.1128/JCM.02316-14. Epub 2014 Oct 1.

Abstract

A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the surveillance of emerging highly virulent strains.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, Bacterial / genetics*
  • Antigens, Surface / genetics*
  • Bacteriological Techniques / methods*
  • Humans
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Molecular Epidemiology / methods
  • Multiplex Polymerase Chain Reaction / methods*
  • Virulence Factors / genetics*

Substances

  • Antigens, Bacterial
  • Antigens, Surface
  • K antigens
  • Virulence Factors