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. 2014 Oct 1;2014(10):pdb.prot080028.
doi: 10.1101/pdb.prot080028.

Purification of Mitochondria by Sucrose Step Density Gradient Centrifugation

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Purification of Mitochondria by Sucrose Step Density Gradient Centrifugation

David A Clayton et al. Cold Spring Harb Protoc. .

Abstract

Mitochondrial fractions isolated from tissue culture cells or tissue such as liver after differential centrifugation can be purified further by density gradient centrifugation. Here we describe the use of sucrose for this purpose because it is commonly used and inexpensive and the resulting mitochondria preparations are useful for many purposes.

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