Endocytosis and recycling of the fibronectin receptor in CHO cells

EMBO J. 1989 May;8(5):1341-8.

Abstract

An anti-fibronectin receptor monoclonal antibody preferentially labels the leading edges of freshly plated CHO fibroblasts, suggesting that this receptor circulates through the endocytic cycle. Using a new labelling reagent, I show that this receptor is indeed endocytosed at 37 degrees C and then returned to the cell surface. These findings imply that fibronectin receptors are recirculated to the leading edge of a motile cell by the endocytic cycle, and establish that the processes of endocytosis/exocytosis and cell locomotion are intimately linked.

MeSH terms

  • Animals
  • Cell Movement
  • Cells, Cultured
  • Dipeptides
  • Endocytosis*
  • Exocytosis
  • Fluorescent Antibody Technique
  • Indicators and Reagents
  • Kinetics
  • Receptors, Fibronectin
  • Receptors, Immunologic / physiology*
  • Succinimides

Substances

  • 3,3'-dithiopropionyl 1-sulfosuccinimidyl 1'-glycyl-tyrosine
  • Dipeptides
  • Indicators and Reagents
  • Receptors, Fibronectin
  • Receptors, Immunologic
  • Succinimides