A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples

Diagn Microbiol Infect Dis. 2014 Dec;80(4):285-6. doi: 10.1016/j.diagmicrobio.2014.09.004. Epub 2014 Sep 16.


We demonstrate the development of a multiplex droplet digital PCR assay for human cytomegalovirus (CMV), human adenovirus species F, and an internal plasmid control that may be useful for PCR inhibition-prone clinical samples. This assay performs better on inhibition-prone stool samples than a quantitative PCR assay for CMV and is the first published clinical virology droplet digital PCR assay to incorporate an internal control.

Keywords: Digital PCR; Inhibition; Quantitative PCR; Stool.

Publication types

  • Comparative Study

MeSH terms

  • Adenoviruses, Human / genetics*
  • Cytomegalovirus / genetics*
  • Cytomegalovirus Infections / virology
  • Feces / virology*
  • Humans
  • Multiplex Polymerase Chain Reaction / methods*
  • Real-Time Polymerase Chain Reaction