Establishment of a Cre recombinase based mutagenesis protocol for markerless gene deletion in Streptococcus suis

J Microbiol Methods. 2014 Dec;107:80-3. doi: 10.1016/j.mimet.2014.09.007. Epub 2014 Oct 1.

Abstract

The lack of knowledge about pathogenicity mechanisms of Streptococcus (S.) suis is, at least partially, attributed to limited methods for its genetic manipulation. Here, we established a Cre-lox based recombination system for markerless gene deletions in S. suis serotype 2 with high selective pressure and without undesired side effects.

Keywords: Cre–lox system; Markerless mutagenesis; Streptococcus suis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Order
  • Genetic Loci
  • Homologous Recombination*
  • Integrases / metabolism*
  • Mutagenesis, Site-Directed / methods*
  • Streptococcus suis / genetics*
  • Streptococcus suis / metabolism*

Substances

  • Cre recombinase
  • Integrases