Establishment of a Cre recombinase based mutagenesis protocol for markerless gene deletion in Streptococcus suis

J Microbiol Methods. 2014 Dec;107:80-3. doi: 10.1016/j.mimet.2014.09.007. Epub 2014 Oct 1.


The lack of knowledge about pathogenicity mechanisms of Streptococcus (S.) suis is, at least partially, attributed to limited methods for its genetic manipulation. Here, we established a Cre-lox based recombination system for markerless gene deletions in S. suis serotype 2 with high selective pressure and without undesired side effects.

Keywords: Cre–lox system; Markerless mutagenesis; Streptococcus suis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Order
  • Genetic Loci
  • Homologous Recombination*
  • Integrases / metabolism*
  • Mutagenesis, Site-Directed / methods*
  • Streptococcus suis / genetics*
  • Streptococcus suis / metabolism*


  • Cre recombinase
  • Integrases