The enduring utility of continuous culturing in experimental evolution
- PMID: 25281774
- PMCID: PMC4411559
- DOI: 10.1016/j.ygeno.2014.09.015
The enduring utility of continuous culturing in experimental evolution
Abstract
Studying evolution in the laboratory provides a means of understanding the processes, dynamics and outcomes of adaptive evolution in precisely controlled and readily replicated conditions. The advantages of experimental evolution are maximized when the selection is well defined, which enables linking genotype, phenotype and fitness. One means of maintaining a defined selection is continuous culturing: chemostats enable the study of adaptive evolution in constant nutrient-limited environments, whereas cells in turbidostats evolve in constant nutrient abundance. Although the experimental effort required for continuous culturing is considerable relative to the experimental simplicity of serial batch culture, the opposite is true of the environments they produce: continuous culturing results in simplified and invariant conditions whereas serially diluted batch cultures are complex and dynamic. The comparative simplicity of the selective environment that is unique to continuous culturing provides an ideal experimental system for addressing key questions in adaptive evolution.
Keywords: Chemostat; Continuous culture; Experimental evolution; Microbial evolution; Turbidostat.
Copyright © 2014 Elsevier Inc. All rights reserved.
Figures
Similar articles
-
The functional basis of adaptive evolution in chemostats.FEMS Microbiol Rev. 2015 Jan;39(1):2-16. doi: 10.1111/1574-6976.12082. Epub 2014 Dec 4. FEMS Microbiol Rev. 2015. PMID: 25098268 Free PMC article. Review.
-
The repertoire and dynamics of evolutionary adaptations to controlled nutrient-limited environments in yeast.PLoS Genet. 2008 Dec;4(12):e1000303. doi: 10.1371/journal.pgen.1000303. Epub 2008 Dec 12. PLoS Genet. 2008. PMID: 19079573 Free PMC article.
-
The omnistat: A flexible continuous-culture system for prolonged experimental evolution.Methods Ecol Evol. 2020 Aug;11(8):932-942. doi: 10.1111/2041-210X.13403. Epub 2020 Jul 5. Methods Ecol Evol. 2020. PMID: 32999708 Free PMC article.
-
Mesostats-A multiplexed, low-cost, do-it-yourself continuous culturing system for experimental evolution of mesocosms.PLoS One. 2022 Jul 28;17(7):e0272052. doi: 10.1371/journal.pone.0272052. eCollection 2022. PLoS One. 2022. PMID: 35901067 Free PMC article.
-
Evolution in the light of fitness landscape theory.Trends Ecol Evol. 2019 Jan;34(1):69-82. doi: 10.1016/j.tree.2018.10.009. Epub 2018 Dec 21. Trends Ecol Evol. 2019. PMID: 30583805 Review.
Cited by
-
"Do It Yourself" Microbial Cultivation Techniques for Synthetic and Systems Biology: Cheap, Fun, and Flexible.Front Microbiol. 2018 Jul 30;9:1666. doi: 10.3389/fmicb.2018.01666. eCollection 2018. Front Microbiol. 2018. PMID: 30105008 Free PMC article.
-
Equilibria and oscillations in cheat-cooperator dynamics.Evol Lett. 2023 Jul 22;7(5):339-350. doi: 10.1093/evlett/qrad032. eCollection 2023 Oct. Evol Lett. 2023. PMID: 37829502 Free PMC article.
-
Intraspecific variation in metal tolerance modulate competition between two marine diatoms.ISME J. 2022 Feb;16(2):511-520. doi: 10.1038/s41396-021-01092-9. Epub 2021 Aug 26. ISME J. 2022. PMID: 34446855 Free PMC article.
-
Salt-stress adaptation of yeast as a simple method to improve high-gravity fermentation in an industrial medium.Appl Microbiol Biotechnol. 2021 Oct;105(20):8009-8018. doi: 10.1007/s00253-021-11566-7. Epub 2021 Sep 23. Appl Microbiol Biotechnol. 2021. PMID: 34553250
-
Multiplexing mutation rate assessment: determining pathogenicity of Msh2 variants in Saccharomyces cerevisiae.Genetics. 2021 Jun 24;218(2):iyab058. doi: 10.1093/genetics/iyab058. Genetics. 2021. PMID: 33848333 Free PMC article.
References
-
- Bell G. Selection. second Oxford Univ Press; 2008.
-
- Gresham D, Ruderfer DM, Pratt SC, Schacherer J, Dunham MJ, Botstein D, et al. Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA microarray. Science. 2006;311:1932–1936. - PubMed
-
- Herring CD, Raghunathan A, Honisch C, Patel T, Applebee MK, Joyce AR, et al. Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale. Nat Genet. 2006;38:1406–1412. - PubMed
Publication types
MeSH terms
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
