MicroRNA-874 inhibits cell proliferation and induces apoptosis in human breast cancer by targeting CDK9

FEBS Lett. 2014 Dec 20;588(24):4527-35. doi: 10.1016/j.febslet.2014.09.035. Epub 2014 Oct 2.


It has been demonstrated that miR-874 plays important roles in many types of cancers. Nevertheless, its biological function in breast cancer remains largely unknown. In this study, we found that the expression level of miR-874 is down-regulated in breast cancer in comparison with the adjacent normal tissues. The overexpression of miR-874 is able to inhibit cell proliferation and induce cell apoptosis and cell cycle arrest in MCF7 and MDA-MB-231 cells. Using a bioinformatics method, we further show that CDK9 is a direct target of miR-874 and that its protein level is negatively regulated by miR-874. Therefore, the data reported in this manuscript demonstrate that miR-874 is an important regulator in breast cancer and imply that the miR-874/CDK9 axis has potential as a therapeutic target for breast cancer.

Keywords: Apoptosis; Breast cancer; CDK9; Cell cycle; Cell proliferation; miR-874.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Apoptosis / genetics*
  • Base Sequence
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology*
  • Cell Cycle / genetics
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Cyclin-Dependent Kinase 9 / deficiency
  • Cyclin-Dependent Kinase 9 / genetics*
  • Down-Regulation / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Knockdown Techniques
  • Humans
  • MicroRNAs / genetics*
  • Middle Aged


  • MIRN874 microRNA, human
  • MicroRNAs
  • Cyclin-Dependent Kinase 9