A novel Nrf2-miR-29-desmocollin-2 axis regulates desmosome function in keratinocytes

Nat Commun. 2014 Oct 6;5:5099. doi: 10.1038/ncomms6099.


The Nrf2 transcription factor controls the expression of genes involved in the antioxidant defense system. Here, we identified Nrf2 as a novel regulator of desmosomes in the epidermis through the regulation of microRNAs. On Nrf2 activation, expression of miR-29a and miR-29b increases in cultured human keratinocytes and in mouse epidermis. Chromatin immunoprecipitation identified the Mir29ab1 and Mir29b2c genes as direct Nrf2 targets in keratinocytes. While binding of Nrf2 to the Mir29ab1 gene activates expression of miR-29a and -b, the Mir29b2c gene is silenced by DNA methylation. We identified desmocollin-2 (Dsc2) as a major target of Nrf2-induced miR-29s. This is functionally important, since Nrf2 activation in keratinocytes of transgenic mice causes structural alterations of epidermal desmosomes. Furthermore, the overexpression of miR-29a/b or knockdown of Dsc2 impairs the formation of hyper-adhesive desmosomes in keratinocytes, whereas Dsc2 overexpression has the opposite effect. These results demonstrate that a novel Nrf2-miR-29-Dsc2 axis controls desmosome function and cutaneous homeostasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line, Tumor
  • Chromatin Immunoprecipitation
  • CpG Islands
  • DNA Methylation
  • Desmocollins / metabolism*
  • Desmosomes / metabolism*
  • Gene Expression Regulation
  • Gene Silencing
  • Homeostasis
  • Humans
  • Keratinocytes / cytology*
  • Membrane Glycoproteins / metabolism
  • Mice
  • Mice, Transgenic
  • MicroRNAs / metabolism*
  • Molecular Sequence Data
  • NF-E2-Related Factor 2 / metabolism*
  • Skin / metabolism


  • DSC2 protein, human
  • Desmocollins
  • Dsc2 protein, mouse
  • MIRN29 microRNA, human
  • MIRN29 microRNA, mouse
  • Membrane Glycoproteins
  • MicroRNAs
  • NF-E2-Related Factor 2
  • NFE2L2 protein, human
  • Nfe2l2 protein, mouse