Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 59 (12), 2927-34

Protective Effects of Garlic Extract, PMK-S005, Against Nonsteroidal Anti-Inflammatory Drugs-Induced Acute Gastric Damage in Rats

Affiliations

Protective Effects of Garlic Extract, PMK-S005, Against Nonsteroidal Anti-Inflammatory Drugs-Induced Acute Gastric Damage in Rats

Yoon Jeong Choi et al. Dig Dis Sci.

Abstract

Background: PMK-S005 is synthetic s-allyl-L-cysteine (SAC), a sulfur-containing amino acid, which was initially isolated from garlic. The antioxidant and anti-inflammation activities of SAC have been demonstrated in diverse experimental animal models.

Aims: The purpose of this study was to investigate the gastroprotective effects of PMK-S005 against NSAIDs-induced acute gastric damage in rats.

Methods: Eight-week SD rats were pretreated with PMK-S005 (1, 5, or 10 mg/kg) or rebamipide (50 mg/kg) 1 h before administration of NSAIDs including aspirin (200 mg/kg), diclofenac (80 mg/kg), and indomethacin (40 mg/kg). After 4 h, the gross ulcer index, histological index, and gastric mucus level were determined. Myeloperoxidase (MPO), TNF-α, IL-1β, PGE2, and LTB4 levels were estimated in the gastric mucosal tissue by ELISA. Protein expressions of cPLA2, COX-1, and COX-2 were assessed by Western blot analysis.

Results: Pretreatment with PMK-S005 significantly attenuated the NSAIDs-induced gastric damage and increased the gastric mucus level. In addition, PMK-S005 attenuated increases in MPO, TNF-α, and IL-1β production. The expressions of cPLA2 and COX-2 induced by NSAIDs were decreased by PMK-S005 pretreatment. PMK-S005 did not cause suppression of PGE2 synthesis induced by NSAIDs, but LTB4 production was significantly suppressed by PMK-S005. The effects of PMK-S005 were consistently maximized at a concentration of 5 mg/kg, which were frequently superior to those of rebamipide.

Conclusions: These results strongly suggest that PMK-S005 can be a useful gastroprotective agent against acute gastric mucosal damage by suppressing proinflammatory cytokines, down-regulating cPLA2, COX-2 and LTB4 expression, and increasing the synthesis of mucus.

Similar articles

See all similar articles

Cited by 6 PubMed Central articles

See all "Cited by" articles

References

    1. J Neurochem. 2011 May;117(3):388-402 - PubMed
    1. Aliment Pharmacol Ther. 2009 Sep 15;30(6):517-31 - PubMed
    1. Gut Liver. 2012 Apr;6(2):210-7 - PubMed
    1. Best Pract Res Clin Gastroenterol. 2010 Apr;24(2):121-32 - PubMed
    1. J Agric Food Chem. 2012 Mar 28;60(12):3158-65 - PubMed

Publication types

MeSH terms

LinkOut - more resources

Feedback