Significance of Mutations in Hepatitis B Virus X Gene for the Pathogenesis of HB-associated Glomerulonephritis

Acta Virol. 2014;58(3):278-81. doi: 10.4149/av_2014_03_278.

Abstract

In this study, the significance of hepatitis virus (HBV) X gene mutations for the pathogenesis of HBV-associated glomerulonephritis (HBV-GN) was investigated. DNA was extracted from 50 HBV-GN patients and 60 asymptomatic HBV carriers and subjected to PCR amplification and sequencing of HBV X gene. In HBV-GN patients, missense nucleotide mutations of C1653T, A1726C, A1727T, C1730G, T1753C, A1762T, and G1764A were detected in 84% of subjects, all located in the trans-acting regulatory region of the X gene. In control patients, missense nucleotide mutations of A1632C and A1635C were detected in 8% of subjects, both located in the non-functional region of the X gene. We conclude that, in most HBV-GN patients, X gene missense mutations occurred at some key sites playing an important role in the pathogenesis of HBV-GN.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Genotype
  • Glomerulonephritis / etiology
  • Glomerulonephritis / virology*
  • Hepatitis B / complications*
  • Hepatitis B / virology
  • Hepatitis B virus / genetics*
  • Hepatitis B virus / metabolism
  • Humans
  • Mutation, Missense*
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism

Substances

  • Trans-Activators
  • hepatitis B virus X protein