The U1 RNA-binding site of the U1 small nuclear ribonucleoprotein (snRNP)-associated A protein suggests a similarity with U2 snRNPs

Mol Cell Biol. 1989 Jul;9(7):2975-82. doi: 10.1128/mcb.9.7.2975-2982.1989.

Abstract

The site of interaction between human U1 RNA and one of its uniquely associated proteins, A, was examined with in vitro binding assays. The A protein bound directly to stem-loop II of U1 RNA in a region which exhibits sequence similarity to U2 RNA. The similarity with U2 RNA was in a region that has been shown to interact with a U2 RNA-associated protein. The A protein-binding site on U1 RNA overlapped a previously described epitope for an RNA-specific human autoantibody (S. L. Deutscher and J. D. Keene, Proc. Natl. Acad. Sci. USA 85:3299-3303, 1988), supporting the hypothesis that the anti-RNA antibody originated as an anti-idiotypic response to A protein-specific autoantibodies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Autoantibodies / metabolism
  • Base Sequence
  • Binding Sites
  • Binding, Competitive
  • Blotting, Northern
  • Blotting, Western
  • Carrier Proteins / metabolism*
  • Escherichia coli / genetics
  • Exoribonucleases
  • Genetic Techniques
  • HeLa Cells
  • Humans
  • Molecular Weight
  • Nucleic Acid Conformation
  • Precipitin Tests
  • RNA-Binding Proteins
  • Ribonucleoproteins / metabolism*
  • Ribonucleoproteins, Small Nuclear

Substances

  • Autoantibodies
  • Carrier Proteins
  • RNA-Binding Proteins
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • Exoribonucleases
  • exoribonuclease T