Nuclear transfer and transgenesis in the pig

Mayuko Kurome; Barbara Kessler; Annegret Wuensch; Hiroshi Nagashima; Eckhard Wolf

doi:10.1007/978-1-4939-1594-1_4

Nuclear transfer and transgenesis in the pig

Methods Mol Biol. 2015:1222:37-59. doi: 10.1007/978-1-4939-1594-1_4.

Authors

Mayuko Kurome¹, Barbara Kessler, Annegret Wuensch, Hiroshi Nagashima, Eckhard Wolf

Affiliation

¹ Chair for Molecular Animal Breeding and Biotechnology, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Feodor-Lynen-Str. 25, 81377, Munich, Germany.

PMID: 25287337
DOI: 10.1007/978-1-4939-1594-1_4

Abstract

Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the generation of tailored pig models for biomedical research and for xenotransplantation. Up to now, SCNT is the main way to generate gene-targeted pigs, since germ line-competent pluripotent stem cells are not available for this species. In this chapter, we introduce our routine workflow for the production of genetically engineered pigs, especially focused on the genetic modification of somatic donor cells, SCNT using in vitro matured oocytes, and laparoscopic embryo transfer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified*
Anti-Bacterial Agents / pharmacology
Embryo Transfer / methods*
Female
Gene Targeting / methods*
Gene Transfer Techniques
In Vitro Oocyte Maturation Techniques / methods*
Laparoscopy / methods
Nuclear Transfer Techniques* / instrumentation
Pregnancy
Sus scrofa / genetics*
Transfection / methods
Workflow

Substances

Anti-Bacterial Agents