Assessment of cell lineages and cell death in blastocysts by immunostaining

Methods Mol Biol. 2015:1222:175-80. doi: 10.1007/978-1-4939-1594-1_13.


During the last decade it has been shown that most mammalian blastocysts consisted of three cell lineages. Immunofluorescence with multiple antibodies enables to identify each cell type allowing an easy detection of eventual defects. It is complementary to RT-PCR experiments as this technique allows to look at cell position and to analyze and count the proportions between the different cell types. Thus after any kind of embryo manipulation such as nuclear transfer (NT), the analysis of the three cell lineages by immunofluorescence will provide criteria for good or poor development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Blastocyst / cytology*
  • CDX2 Transcription Factor
  • Cell Death
  • Cell Lineage
  • GATA6 Transcription Factor / immunology
  • Homeodomain Proteins / immunology
  • Immunohistochemistry*
  • Mice
  • Molecular Imaging / instrumentation
  • Molecular Imaging / methods
  • Nanog Homeobox Protein
  • SOXF Transcription Factors / immunology
  • Transcription Factors / immunology


  • Biomarkers
  • CDX2 Transcription Factor
  • Cdx2 protein, mouse
  • GATA6 Transcription Factor
  • Gata6 protein, mouse
  • Homeodomain Proteins
  • Nanog Homeobox Protein
  • Nanog protein, mouse
  • SOX17 protein, human
  • SOXF Transcription Factors
  • Transcription Factors