Poly (lactic acid)/chitosan fiber mats: investigation of effects of the support on lipase immobilization

Nataly M Siqueira; Ketlin C Garcia; Roberta Bussamara; Fernanda S Both; Marilene H Vainstein; Rosane M D Soares

doi:10.1016/j.ijbiomac.2014.08.048

Poly (lactic acid)/chitosan fiber mats: investigation of effects of the support on lipase immobilization

Int J Biol Macromol. 2015 Jan:72:998-1004. doi: 10.1016/j.ijbiomac.2014.08.048. Epub 2014 Oct 5.

Authors

Nataly M Siqueira¹, Ketlin C Garcia², Roberta Bussamara³, Fernanda S Both³, Marilene H Vainstein³, Rosane M D Soares⁴

Affiliations

¹ Laboratory of Advanced Polymers, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Lab K-208 A, Porto Alegre, RS CEP 91501-970, Brazil; Post-Graduate Program of Materials Science, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Sala A216, Porto Alegre, RS CEP 91501-970, Brazil.
² Laboratory of Advanced Polymers, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Lab K-208 A, Porto Alegre, RS CEP 91501-970, Brazil.
³ Laboratory of Fungi Importance Medicine and Biotechnology, Institute of Biotechnology, Universidade Federal do Rio Grande do Sul, Lab 220, Av. Bento Gonçalves, 9500, Porto Alegre, RS CEP 91501-970, Brazil.
⁴ Laboratory of Advanced Polymers, Institute of Chemistry, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Lab K-208 A, Porto Alegre, RS CEP 91501-970, Brazil; Post-Graduate Program of Materials Science, Universidade Federal do Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Sala A216, Porto Alegre, RS CEP 91501-970, Brazil. Electronic address: soaresr@ufrgs.br.

PMID: 25290984
DOI: 10.1016/j.ijbiomac.2014.08.048

Abstract

The greatest challenge for biotechnological processes is to have immobilized enzymes acting as good green catalysts with high reusability rates. In this work, we have produced electrospun fibers from poly (lactic acid)/chitosan blends. Further, we evaluated the influence of these materials as support for lipase immobilization. The PLA/chitosan fiber mats were composed by non-woven nanofibers, with diameters ranging from 200 nm to 1.3 μm. The solvent (TFA) as well as the chitosan addition influenced morphology, hydrophobicity and mechanical properties of PLA nanofibers. It was observed that even for lower concentration of lipase (5 U) higher enzyme activity retention was detected in the PLA/chitosan blends. In addition, a remarkable improvement of lipase activity on pure PLA fiber mat was verified, indicating that most of the enzymes were probably in their active form.

Keywords: Chitosan; Electrospinning; Immobilization; Lipase; Nanofibers; poly (lactic acid).

MeSH terms

Chitosan / chemical synthesis
Chitosan / chemistry*
Enzymes, Immobilized / chemistry
Lactic Acid / chemical synthesis
Lactic Acid / chemistry*
Lipase / chemistry*
Lipase / metabolism
Nanofibers / chemistry*
Polyesters
Polymers / chemical synthesis
Polymers / chemistry*
Surface Properties
Tensile Strength / drug effects
Tissue Engineering

Substances

Enzymes, Immobilized
Polyesters
Polymers
Lactic Acid
poly(lactide)
Chitosan
Lipase