Abstract
Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. Here the solution structure of the forkhead DNA binding domain of Brugia malayi DAF-16a, a putative ortholog of Caenorhabditis elegans DAF-16, is reported. It is believed to be the first structure of a forkhead or winged helix domain from an invertebrate. C. elegans DAF-16 is involved in the insulin/IGF-I signaling pathway and helps control metabolism, longevity, and development. Conservation of sequence and structure with human FOXO proteins suggests that B. malayi DAF-16a is a member of the FOXO family of forkhead proteins.
Keywords:
Brugia malayi; Caenorhabditis elegans; DAF-16; FOXO; FOXO1; FOXO3a; FOXO4; filariasis; forkhead box; insulin-signaling pathway; winged helix.
© 2014 Wiley Periodicals, Inc.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Binding Sites
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Brugia malayi / metabolism*
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Caenorhabditis elegans Proteins / chemistry
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Caenorhabditis elegans Proteins / metabolism
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Conserved Sequence
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DNA / chemistry*
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DNA / metabolism
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Electrophoretic Mobility Shift Assay
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Forkhead Transcription Factors / chemistry*
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Forkhead Transcription Factors / genetics
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Forkhead Transcription Factors / metabolism
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Helminth Proteins / chemistry*
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Helminth Proteins / genetics
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Helminth Proteins / metabolism
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Models, Molecular*
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Molecular Sequence Data
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Molecular Weight
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Peptide Fragments / chemistry*
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Peptide Fragments / genetics
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Peptide Fragments / metabolism
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Protein Conformation
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Protein Structure, Tertiary
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Response Elements
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Sequence Alignment
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Solubility
Substances
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Caenorhabditis elegans Proteins
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Forkhead Transcription Factors
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Helminth Proteins
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Peptide Fragments
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Recombinant Proteins
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daf-16 protein, C elegans
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DNA