Lactobacilli-fermented Hwangryunhaedoktang has enhanced anti-inflammatory effects mediated by the suppression of MAPK signaling pathway in LPS-stimulated RAW 264.7 cells

You-Chang Oh; Yun Hee Jeong; Won-Kyung Cho; Kwang Jin Lee; Taesoo Kim; Jin Yeul Ma

doi:10.4103/0973-1296.139815

Lactobacilli-fermented Hwangryunhaedoktang has enhanced anti-inflammatory effects mediated by the suppression of MAPK signaling pathway in LPS-stimulated RAW 264.7 cells

Pharmacogn Mag. 2014 Aug;10(Suppl 3):S645-54. doi: 10.4103/0973-1296.139815.

Authors

You-Chang Oh¹, Yun Hee Jeong¹, Won-Kyung Cho¹, Kwang Jin Lee¹, Taesoo Kim¹, Jin Yeul Ma¹

Affiliation

¹ Korean Medicine-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, Jeonmin dong, Yuseong, Daejeon, 305-811, Korea.

Abstract

Background: Hwangryunhaedoktang (HR) has been traditionally used in oriental medicine as a drug for the treatment of melena, hemoptysis, and apoplexy.

Objective: We investigated whether HR and lactobacilli-fermented HRs have an inhibitory effect on the production of proinflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells.

Materials and methods: The investigation was focused on whether HR and fermented HRs could inhibit the production of prostaglandin (PG)E2, nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-6, cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) and mitogen-activated protein kinases (MAPKs) in LPS-stimulated RAW 264.7 cells.

Results: We found that HR weakly inhibited various inflammatory mediators induced by LPS. However, fermentation with lactobacilli significantly increased the inhibitory effect of HR on most of the inflammatory mediator expression. Furthermore, fermented HRs exerted a stronger inhibitory effect on MAPKs phosphorylation than that by non-fermented HR.

Conclusions: These results suggest that lactobacilli-fermented HRs contains elevated potent anti-inflammatory activity that is mediated by inhibiting MAPKs pathway in macrophages.

Keywords: Hwangryunhaedoktang and fermented Hwangryunhaedoktang; cyclooxygenase 2; inducible nitric oxide synthase; inflammatory cytokines; mitogen-activated protein kinases.