Elevated C-reactive protein levels and enhanced high frequency vasomotion in patients with ischemic heart disease during brachial flow-mediated dilation

PLoS One. 2014 Oct 9;9(10):e110013. doi: 10.1371/journal.pone.0110013. eCollection 2014.


Purpose: The physiological role of vasomotion, rhythmic oscillations in vascular tone or diameter, and its underlying mechanisms are unknown. We investigated the characteristics of brachial artery vasomotion in patients with ischemic heart disease (IHD).

Methods: We performed a retrospective study of 37 patients with IHD. Endothelial function was assessed using flow-mediated dilation (FMD), and power spectral analysis of brachial artery diameter oscillations during FMD was performed. Frequency-domain components were calculated by integrating the power spectrums in three frequency bands (in ms2) using the MemCalc (GMS, Tokyo, Japan): very-low frequency (VLF), 0.003-0.04 Hz; low frequency (LF), 0.04-0.15 Hz; and high frequency (HF), 0.15-0.4 Hz. Total spectral power (TP) was calculated as the sum of all frequency bands, and each spectral component was normalized against TP.

Results: Data revealed that HF/TP closely correlated with FMD (r = -0.33, p = 0.04), whereas VLF/TP and LF/TP did not. We also explored the relationship between elevated C-reactive protein (CRP) levels and vasomotion. HF/TP was significantly increased in subjects with high CRP levels (CRP;>0.08 mg/dL) compared with subjects with low CRP levels (0.052±0.026 versus 0.035±0.022, p<0.05). The HF/TP value closely correlated with CRP (r = 0.24, p = 0.04), whereas the value of FMD did not (r = 0.023, p = 0.84). In addition, elevated CRP levels significantly increased the value of HF/TP after adjustment for FMD and blood pressure (β = 0.33, p<0.05).

Conclusion: The HF component of brachial artery diameter oscillation during FMD measurement correlated well with FMD and increased in the presence of elevated CRP levels in subjects with IHD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Autonomic Nervous System / metabolism
  • Autonomic Nervous System / physiopathology
  • Blood Pressure
  • Brachial Artery / metabolism
  • Brachial Artery / physiopathology*
  • C-Reactive Protein / metabolism*
  • Electrocardiography
  • Female
  • Heart Rate
  • Hemoglobins / metabolism
  • Humans
  • Japan
  • Male
  • Middle Aged
  • Myocardial Ischemia / blood*
  • Myocardial Ischemia / physiopathology
  • Tokyo
  • Vasomotor System / metabolism*
  • Vasomotor System / physiopathology


  • Hemoglobins
  • C-Reactive Protein

Grant support

This research was supported by the Japan Society for the Promotion of Science through the Funding Program for World-Leading Innovative R&D on Science and Technology program, research grants from the Fukuda Foundation for Medical Technology (to E.A.), and Ministry of Education, Culture, Sports, Science, and Technology of Japan Grant-in-aid 23790837 (to E. A.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.