Litorivivens lipolytica gen. nov., sp. nov., a lipolytic bacterium isolated from a tidal flat

Int J Syst Evol Microbiol. 2015 Jan;65(Pt 1):141-146. doi: 10.1099/ijs.0.069500-0. Epub 2014 Oct 9.


A Gram-stain-negative, flagellated, aerobic and rod-shaped or ovoid bacterial strain, designated HJTF-7(T), was isolated from a tidal flat on the South Sea of South Korea, and its taxonomic position was investigated using a polyphasic approach. Strain HJTF-7(T) grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0% (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees, based on 16S rRNA gene sequences, showed that strain HJTF-7(T) joined the cluster comprising the type strains of species of the genera Spongiibacter and Zhongshania. Strain HJTF-7(T) exhibited 16S rRNA gene sequence similarities of 90.4-92.5% to the type strains of species of the genera Spongiibacter and Zhongshania and of less than 91.5% to the type strains of other recognized species. Strain HJTF-7(T) contained Q-8 as the predominant ubiquinone. The major fatty acids were iso-C17:1ω9c, iso-C15:0, iso-C17:0, iso-C11:0 3-OH and C17:1ω8c and the major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The fatty acid and polar lipid profiles of strain HJTF-7(T) were distinct from those of members of the genera Spongiibacter and Zhongshania. The DNA G+C content of strain HJTF-7(T) was 55.9 mol%. The phylogenetic data and differential chemotaxonomic and other phenotypic properties revealed that strain HJTF-7(T) represents a novel genus and species within the class Gammaproteobacteria, for which the name Litorivivens lipolytica gen. nov., sp. nov. is proposed. The type strain of Litorivivens lipolytica is HJTF-7(T) ( =KCTC 42157(T) =CECT 8654(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Bacterial / genetics
  • Fatty Acids / chemistry
  • Gammaproteobacteria / classification*
  • Gammaproteobacteria / genetics
  • Gammaproteobacteria / isolation & purification
  • Geologic Sediments / microbiology*
  • Molecular Sequence Data
  • Phosphatidylethanolamines / chemistry
  • Phosphatidylglycerols / chemistry
  • Phylogeny*
  • RNA, Ribosomal, 16S / genetics
  • Republic of Korea
  • Seawater / microbiology*
  • Sequence Analysis, DNA
  • Ubiquinone / chemistry


  • DNA, Bacterial
  • Fatty Acids
  • Phosphatidylethanolamines
  • Phosphatidylglycerols
  • RNA, Ribosomal, 16S
  • Ubiquinone
  • phosphatidylethanolamine
  • ubiquinone 8

Associated data

  • GENBANK/KM017973