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Comparative Study
. 2014 Oct 10;7:714.
doi: 10.1186/1756-0500-7-714.

Harvesting the Maximum Length of Sciatic Nerve From Adult Mice: A Step-By-Step Approach

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Free PMC article
Comparative Study

Harvesting the Maximum Length of Sciatic Nerve From Adult Mice: A Step-By-Step Approach

Usman Bala et al. BMC Res Notes. .
Free PMC article

Abstract

Background: Over the past several decades, many studies concerning peripheral nerve damage or regeneration have been performed. Mice have been widely used for many of these studies, with the sciatic nerve being the most targeted and preferred nerve. Therefore, techniques for harvesting mouse sciatic nerves of a maximum length that is sufficient for different analyses will be highly valuable. Here we describe a simple step-by-step guide for harvesting the maximum length of mouse sciatic nerve and compare the length of the harvested nerves gathered with the proposed method with nerves obtained using a conventional mid-thigh incision approach.

Findings: The sciatic nerve was exposed while holding both hind limbs together in one hand and the tail was gently pulled away in the opposite direction. The nerve was traced by dissecting through its course both distally and proximally and was carefully harvested. The total average length of the sciatic nerves obtained using the proposed harvesting method and the mid-thigh incision method was 22.60 ± 1.62 mm and 7.0 ± 0.76 mm, respectively. This length of harvested nerve allows further dissection into several segments that can be used for additional independent analyses such as histochemical/histological analysis and RNA or protein extraction.

Conclusion: The approach described here has several advantages over mid-thigh incision methods in that it: i) allows harvesting of maximum lengths of the sciatic nerve ii) allows simultaneous harvesting of both sciatic nerves, iii) provides time savings; iv) requires no extensive knowledge of veterinary anatomy; and v) provides hassle-free dissection.

Figures

Figure 1
Figure 1
Step-by-step approach for harvesting the sciatic nerve. Mouse preparation and euthanasia (a). Exposure of hind limb muscles and posterior abdominal wall (b-e). Exposure of the sciatic nerve by pulling method (f, g). Tracing and harvesting the sciatic nerve (h, i). APV = Abdomino-pelvic viscera; BHL = Bent hind limb; HL = Hind limb; PAW = Posterior abdominal wall; SN = Sciatic nerve; T = Tail; UO = Urethral opening; XP = Xiphoid process; White dotted line = incision lines; Black dotted lines = small cut.
Figure 2
Figure 2
Measurement of representative sciatic nerves harvested via the proposed method (a-b) and conventional mid-thigh incision approach (c). The point of bifurcation into tibial and common peroneal nerves is indicated by arrows (a-b).

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