Microbiologic methods utilized in the MAL-ED cohort study

Clin Infect Dis. 2014 Nov 1;59 Suppl 4(Suppl 4):S225-32. doi: 10.1093/cid/ciu413.


A central hypothesis of The Etiology, Risk Factors and Interactions of Enteric Infections and Malnutrition and the Consequences for Child Health and Development (MAL-ED) study is that enteropathogens contribute to growth faltering. To examine this question, the MAL-ED network of investigators set out to achieve 3 goals: (1) develop harmonized protocols to test for a diverse range of enteropathogens, (2) provide quality-assured and comparable results from 8 global sites, and (3) achieve maximum laboratory throughput and minimum cost. This paper describes the rationale for the microbiologic assays chosen and methodologies used to accomplish the 3 goals.

Keywords: ELISA; PCR; culture; enteropathogen; microscopy.

MeSH terms

  • Child, Preschool
  • Enterobacteriaceae Infections / diagnosis
  • Enzyme-Linked Immunosorbent Assay
  • Epidemiologic Research Design*
  • Humans
  • Infant
  • Infant, Newborn
  • Intestinal Diseases / diagnosis
  • Intestinal Diseases, Parasitic / diagnosis
  • Longitudinal Studies
  • Microbiological Techniques / methods*
  • Microbiological Techniques / standards*
  • Microscopy
  • Polymerase Chain Reaction
  • Quality Assurance, Health Care