First report on the antibody verification of HLA-DR, HLA-DQ and HLA-DP epitopes recorded in the HLA Epitope Registry

Rene J Duquesnoy; Marilyn Marrari; Anat R Tambur; Arend Mulder; Luiz Cláudio Demes da Mata Sousa; Adalberto Socorro da Silva; Semiramis J H do Monte

doi:10.1016/j.humimm.2014.09.012

First report on the antibody verification of HLA-DR, HLA-DQ and HLA-DP epitopes recorded in the HLA Epitope Registry

Hum Immunol. 2014 Nov;75(11):1097-103. doi: 10.1016/j.humimm.2014.09.012. Epub 2014 Oct 13.

Authors

Rene J Duquesnoy¹, Marilyn Marrari², Anat R Tambur³, Arend Mulder⁴, Luiz Cláudio Demes da Mata Sousa⁵, Adalberto Socorro da Silva⁵, Semiramis J H do Monte⁶

Affiliations

¹ Division of Transplant Pathology, University of Pittsburgh Medical Center, USA. Electronic address: Duquesnoyr@upmc.edu.
² Division of Transplant Pathology, University of Pittsburgh Medical Center, USA.
³ Transplant Immunology Laboratory, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.
⁴ Department of Immunohematology, Leiden University Medical Center, The Netherlands.
⁵ Department of Informatics and Statistics, Federal University of Piauí, Teresina, Brazil.
⁶ Immunogenetics and Molecular Biology Laboratory, Federal University of Piauí, Teresina, Brazil.

PMID: 25305456
DOI: 10.1016/j.humimm.2014.09.012

Abstract

The International Registry of Antibody-Defined HLA Epitopes (http://www.epregistry.com.br) has been recently established as a tool to understand humoral responses to HLA mismatches. These epitopes can be structurally defined as eplets by three-dimensional molecular modeling and amino acid sequence differences between HLA antigens. A major goal is to identify HLA eplets that have been verified experimentally with informative antibodies. This report addresses class II epitopes encoded by genes in the HLA-D region. Our analysis included reviews of many publications about epitope specificity of class II reactive human and murine monoclonal antibodies and informative alloantibodies from HLA sensitized patients as well as our own antibody testing results. As of July 1, 2014, 24 HLA-DRB1/3/4/5, 15 DQB, 3 DQA and 8 DPB antibody-verified epitopes have been identified and recorded. The Registry is still a work-in-progress and will become a useful resource for HLA professionals interested in histocompatibility testing at the epitope level and investigating antibody responses to HLA mismatches in transplant patients.

Keywords: HLA epitopes; HLA-DP epitopes; HLA-DQ epitopes; HLA-DRB epitopes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Americas
Animals
Antibodies, Monoclonal / chemistry
Epitopes / chemistry
Epitopes / immunology*
Europe
HLA-DP Antigens / chemistry
HLA-DP Antigens / immunology*
HLA-DQ Antigens / chemistry
HLA-DQ Antigens / immunology*
HLA-DR Antigens / chemistry
HLA-DR Antigens / immunology*
Histocompatibility Testing
Humans
International Cooperation
Isoantibodies / chemistry
Mice
Registries*

Substances

Antibodies, Monoclonal
Epitopes
HLA-DP Antigens
HLA-DQ Antigens
HLA-DR Antigens
Isoantibodies