Isolation and identification of aromatic compounds in Lion's Mane Mushroom and their anticancer activities

Food Chem. 2015 Mar 1:170:336-42. doi: 10.1016/j.foodchem.2014.08.078. Epub 2014 Aug 23.


Lion's Mane Mushroom (Hericium erinaceum) is a traditional edible mushroom widely used in culinary applications and as an herbal medicine in East Asian countries. In the present study, two new aromatic compounds, hericerin A (1) and isohericenone J (5), along with five known compounds, isoericerin (2), hericerin (3), N-De phenylethyl isohericerin (4), hericenone J (6), and 4-[3',7'-dimethyl-2',6'-octadienyl]-2-formyl-3-hydroxy-5-methyoxybenzylalcohol (7), were isolated from a methanol extract of the fruiting bodies of H. erinaceum. The chemical structures of the compounds were determined from mass spectra and 1D- and 2D NMR spectroscopy. The anticancer effects of the isolated compounds were examined in HL-60 human acute promyelocytic leukaemia cells. Hericerin A (1) and hericerin (3) significantly reduced cell proliferation with IC50 values of 3.06 and 5.47 μM, respectively. These same compounds also induced apoptosis of HL-60 cells, accompanied by time-dependent down-regulation of p-AKT and c-myc levels. These data suggest that compounds 1 and 3 from H. erinaceum are suitable for use in potential cancer treatments.

Keywords: Anticancer activity; Apoptosis; Hericerin A; Hericiaceae; Hericium erinaceum; Isohericenone J.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales / chemistry*
  • Animals
  • Antineoplastic Agents / therapeutic use*
  • Apoptosis
  • Cell Proliferation / drug effects
  • Humans
  • Phytotherapy / methods*


  • Antineoplastic Agents