Bactericidal properties and biocompatibility of a gentamicin-loaded Fe3O4/carbonated hydroxyapatite coating

Bo Tian; Sha Tang; Chuan-Dong Wang; Wen-Gang Wang; Chuan-Long Wu; Ya-Jun Guo; Ya-Ping Guo; Zhen-An Zhu

doi:10.1016/j.colsurfb.2014.09.050

Bactericidal properties and biocompatibility of a gentamicin-loaded Fe3O4/carbonated hydroxyapatite coating

Colloids Surf B Biointerfaces. 2014 Nov 1:123:403-12. doi: 10.1016/j.colsurfb.2014.09.050. Epub 2014 Oct 5.

Authors

Bo Tian¹, Sha Tang², Chuan-Dong Wang³, Wen-Gang Wang¹, Chuan-Long Wu¹, Ya-Jun Guo², Ya-Ping Guo⁴, Zhen-An Zhu⁵

Affiliations

¹ Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
² The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China.
³ The Key Laboratory of Stem Cell Biology, Shanghai Jiao Tong University School of Medicine (SJTUSM) and Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences (CAS), Shanghai 200025, PR China.
⁴ The Education Ministry Key Lab of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Shanghai Normal University, Shanghai 200234, China. Electronic address: ypguo@shnu.edu.cn.
⁵ Shanghai Key Laboratory of Orthopedic Implant, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China. Electronic address: zhuzhenan2006@126.com.

PMID: 25306864
DOI: 10.1016/j.colsurfb.2014.09.050

Abstract

Postoperative implant-associated infection remains a serious complication in total joint arthroplasty (TJA) surgery. The addition of antibiotics to bone cement is used as an antimicrobial prophylaxis in cemented joint arthroplasty; however, in cementless arthroplasty, there are no comparable measures for the local delivery of antibiotics. In this study, a gentamicin-loaded Fe3O4/carbonated hydroxyapatite coating (Gent-MCHC) was fabricated according to the following steps: (i) deposition of Fe3O4/CaCO3 particles on Ti6Al4V substrates by electrophoretic deposition; (ii) conversions of MCHC from Fe3O4/CaCO3 coatings by chemical treatment; and (iii) formation of Gent-MCHC by loading gentamicin into MCHC. MCHC possessed mesoporous structure with a pore size of about 3.8 nm and magnetic property with the saturation magnetization strength of about 4.03 emu/g. Gent-MCHC had higher drug loading efficiency and drug release capacity, and superior biocompatibility and mitogenic activity than Ti6Al4V. Moreover, Gent-MCHC deterred bacterial adhesion and prevented biofilm formation. These results demonstrate that Gent-MCHC can be used as a local drug delivery system to prevent implant-associated infection in TJA surgery.

Keywords: Biocoating; Biofilm prevention; Cementless arthroplasty; Drug delivery system; Implant-associated infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / chemistry*
Anti-Bacterial Agents / pharmacology*
Biocompatible Materials / chemistry*
Biocompatible Materials / pharmacology*
Drug Delivery Systems / adverse effects
Durapatite / chemistry*
Ferric Compounds / chemistry*
Gentamicins / chemistry*
Gentamicins / pharmacology*
Materials Testing
Porosity
Staphylococcus aureus / drug effects

Substances

Anti-Bacterial Agents
Biocompatible Materials
Ferric Compounds
Gentamicins
ferric oxide
Durapatite