Advances in the directed evolution of proteins

Michael D Lane; Burckhard Seelig

doi:10.1016/j.cbpa.2014.09.013

Advances in the directed evolution of proteins

Curr Opin Chem Biol. 2014 Oct:22:129-36. doi: 10.1016/j.cbpa.2014.09.013. Epub 2014 Oct 11.

Authors

Michael D Lane¹, Burckhard Seelig²

Affiliations

¹ Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA; BioTechnology Institute, University of Minnesota, St. Paul, MN, USA.
² Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA; BioTechnology Institute, University of Minnesota, St. Paul, MN, USA. Electronic address: seelig@umn.edu.

Abstract

Natural evolution has produced a great diversity of proteins that can be harnessed for numerous applications in biotechnology and pharmaceutical science. Commonly, specific applications require proteins to be tailored by protein engineering. Directed evolution is a type of protein engineering that yields proteins with the desired properties under well-defined conditions and in a practical time frame. While directed evolution has been employed for decades, recent creative developments enable the generation of proteins with previously inaccessible properties. Novel selection strategies, faster techniques, the inclusion of unnatural amino acids or modifications, and the symbiosis of rational design approaches and directed evolution continue to advance protein engineering.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Animals
Directed Molecular Evolution / methods*
Humans
Models, Molecular
Protein Engineering / methods*
Proteins / chemistry
Proteins / genetics*

Substances

Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding