Detection of avian-like rotavirus A VP4 from a calf in Japan

J Vet Med Sci. 2015 Feb;77(2):221-4. doi: 10.1292/jvms.14-0379. Epub 2014 Oct 13.

Abstract

A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011-2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. Phylogenetic analysis also revealed a close genetic relationship between N2342 and avian RVAs, suggesting bird-to-cattle transmission. We observed frequent contact of wild birds with calves in the farm, suggesting that these birds were the source of the virus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Capsid Proteins / genetics
  • Capsid Proteins / isolation & purification*
  • Capsid Proteins / metabolism
  • Cattle
  • Cattle Diseases / virology*
  • Gene Expression Regulation, Viral / physiology
  • Japan / epidemiology
  • Phylogeny
  • Rotavirus / classification*
  • Rotavirus Infections / epidemiology
  • Rotavirus Infections / veterinary*
  • Rotavirus Infections / virology

Substances

  • Capsid Proteins
  • VP4 protein, Rotavirus