Integrity of the exon 6 sequence is essential for tissue-specific alternative splicing of human leukocyte common antigen pre-mRNA

Mol Cell Biol. 1989 Oct;9(10):4550-5. doi: 10.1128/mcb.9.10.4550-4555.1989.


By alternative splicing, exons 4, 5, and 6 of the human leukocyte common antigen (LCA) gene are included in B-cell mRNA but excluded from thymocyte mRNA. A mini-LCA gene that contains only LCA exons 2, 6, and 8 faithfully reproduces this tissue-specific alternative splicing in mouse B and thymocyte cell lines. Elimination of almost all of the intron sequences associated with exon 6 had no effect on the alternative splicing, while linker-scanning analysis showed that a significant length of the exon 6 sequence is essential for alternative splicing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, Differentiation / genetics*
  • B-Lymphocytes / analysis
  • Base Sequence
  • DNA Mutational Analysis
  • Exons / physiology*
  • Genetic Vectors
  • Histocompatibility Antigens / genetics*
  • Humans
  • Introns / physiology
  • Leukocyte Common Antigens
  • Molecular Sequence Data
  • RNA Splicing / genetics*
  • RNA, Messenger / analysis
  • T-Lymphocytes / analysis
  • Transfection


  • Antigens, Differentiation
  • Histocompatibility Antigens
  • RNA, Messenger
  • Leukocyte Common Antigens

Associated data

  • GENBANK/M29253