Abstract
By alternative splicing, exons 4, 5, and 6 of the human leukocyte common antigen (LCA) gene are included in B-cell mRNA but excluded from thymocyte mRNA. A mini-LCA gene that contains only LCA exons 2, 6, and 8 faithfully reproduces this tissue-specific alternative splicing in mouse B and thymocyte cell lines. Elimination of almost all of the intron sequences associated with exon 6 had no effect on the alternative splicing, while linker-scanning analysis showed that a significant length of the exon 6 sequence is essential for alternative splicing.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, Differentiation / genetics*
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B-Lymphocytes / analysis
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Base Sequence
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DNA Mutational Analysis
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Exons / physiology*
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Genetic Vectors
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Histocompatibility Antigens / genetics*
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Humans
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Introns / physiology
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Leukocyte Common Antigens
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Molecular Sequence Data
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RNA Splicing / genetics*
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RNA, Messenger / analysis
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T-Lymphocytes / analysis
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Transfection
Substances
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Antigens, Differentiation
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Histocompatibility Antigens
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RNA, Messenger
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Leukocyte Common Antigens