Fragment-based screening of the bromodomain of ATAD2

J Med Chem. 2014 Nov 26;57(22):9687-92. doi: 10.1021/jm501035j. Epub 2014 Nov 11.


Cellular and genetic evidence suggest that inhibition of ATAD2 could be a useful strategy to treat several types of cancer. To discover small-molecule inhibitors of the bromodomain of ATAD2, we used a fragment-based approach. Fragment hits were identified using NMR spectroscopy, and ATAD2 was crystallized with three of the hits identified in the fragment screen.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • ATPases Associated with Diverse Cellular Activities
  • Adenosine Triphosphatases / chemistry*
  • Antineoplastic Agents / chemistry
  • Binding Sites
  • Chemistry, Pharmaceutical / methods*
  • Crystallography, X-Ray / methods
  • DNA-Binding Proteins / chemistry*
  • Humans
  • Kinetics
  • Ligands
  • Magnetic Resonance Spectroscopy / methods
  • Molecular Conformation
  • Neoplasms / drug therapy
  • Protein Structure, Tertiary


  • Antineoplastic Agents
  • DNA-Binding Proteins
  • Ligands
  • Adenosine Triphosphatases
  • ATAD2 protein, human
  • ATPases Associated with Diverse Cellular Activities