Phosphoproteomics Reveals the Effect of Ethylene in Soybean Root Under Flooding Stress

J Proteome Res. 2014 Dec 5;13(12):5618-34. doi: 10.1021/pr500621c. Epub 2014 Oct 24.

Abstract

Flooding has severe negative effects on soybean growth. To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each time point. After 3 h of flooding exposure, the fresh weight of soybeans increased, whereas the ATP content of soybean root tips decreased. Using a gel-free proteomic technique, a total of 114 phosphoproteins were identified in the root tip samples, and 34 of the phosphoproteins were significantly changed with respect to phosphorylation status after 3 h of flooding stress. Among these phosphoproteins, eukaryotic translation initiation factors were dephosphorylated, whereas several protein synthesis-related proteins were phosphorylated. The mRNA expression levels of sucrose phosphate synthase 1F and eukaryotic translation initiation factor 4 G were down-regulated, whereas UDP-glucose 6-dehydrogenase mRNA expression was up-regulated during growth but down-regulated under flooding stress. Furthermore, bioinformatic protein interaction analysis of flooding-responsive proteins based on temporal phosphorylation patterns indicated that eukaryotic translation initiation factor 4 G was located in the center of the network during flooding. Soybean eukaryotic translation initiation factor 4 G has homology to programmed cell death 4 protein and is implicated in ethylene signaling. The weight of soybeans was increased with treatment by an ethylene-releasing agent under flooding condition, but it was decreased when plants were exposed to an ethylene receptor antagonist. These results suggest that the ethylene signaling pathway plays an important role, via the protein phosphorylation, in mechanisms of plant tolerance to the initial stages of flooding stress in soybean root tips.

Keywords: flooding stress; phosphoproteomics; root tip; soybean.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological
  • Energy Metabolism
  • Ethylenes / metabolism*
  • Floods
  • Gene Expression Regulation, Plant
  • Organ Specificity
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Plant Proteins / genetics
  • Plant Proteins / metabolism*
  • Plant Roots / growth & development
  • Plant Roots / metabolism*
  • Protein Biosynthesis
  • Protein Processing, Post-Translational
  • Proteome / genetics
  • Proteome / metabolism*
  • Proteomics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Signal Transduction
  • Soybeans / growth & development
  • Soybeans / metabolism*
  • Stress, Physiological

Substances

  • Ethylenes
  • Phosphoproteins
  • Plant Proteins
  • Proteome
  • RNA, Messenger
  • ethylene