The Kalirin Gene rs9289231 Polymorphism as a Novel Predisposing Marker for Coronary Artery Disease

Lab Med. Fall 2014;45(4):302-8. doi: 10.1309/LMLS813ZDPHRFLUU.


Background: Atherosclerosis is the leading cause of death and disability worldwide. Genetic variations play a major role in the process of atherosclerosis. Recently, rs9289231 genetic variations of the Kalirin gene (KALRN) on chromosome 3q21.2 have been introduced as potential genetic markers for coronary artery disease (CAD).

Objective: In this case-control study, we investigated the association between genetic susceptibility to CAD and rs9289231 G/T polymorphism, located on the KALRN gene, in an Iranian population.

Methods: Our cohort consisted of 1486 individuals undergoing coronary angiography. Of these, we considered the 1007 patients with CAD to be case individuals and the 479 individuals with normal coronary conditions to be control individuals. We performed single-nucleotide polymorphism (SNP) genotyping via the high resolution melting (HRM) technique.

Results: Our data showed that the minor allele (G) frequency of rs9289231 SNP was higher in our CAD group than that in our control group (odds ratio, 1:37; confidence interval, 1.07-1.74; P = .01). The results of our data analysis highlighted a genetic association between rs9289231 polymorphism and severity and development of CAD.

Conclusions: We consider the GG genotype and the G allele of rs9289231 polymorphism of KALRN to be genetic risk factors for CAD in an Iranian population, especially in early-stage atherosclerotic vascular disease.

Keywords: KALRN; coronary artery disease; high resolution melting; rs9289231 polymorphism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Case-Control Studies
  • Chromosomes, Human, Pair 3
  • Coronary Artery Disease / genetics*
  • Female
  • Gene Frequency
  • Genetic Markers*
  • Guanine Nucleotide Exchange Factors / genetics*
  • Humans
  • Iran
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Protein-Serine-Threonine Kinases / genetics*


  • Genetic Markers
  • Guanine Nucleotide Exchange Factors
  • KALRN protein, human
  • Protein-Serine-Threonine Kinases