Abstract
RNA interference (RNAi) technology is a promising approach for efficient silencing of a particular gene for cancer gene therapy. However, the main obstacle for the development of RNAi-based therapeutic approaches is the delivery of the RNAi effector molecules to target cells. One promising strategy to surmount this challenge is the application of nonpathogenic bacteria as a delivery vector to target cells. In this chapter, the design of invasive Escherichia coli is described. The strain carries a plasmid encoding short hairpin RNAs (shRNAs), a protein (invasin) necessary for endocytotic absorption of the bacteria by target cells, and listeriolysin O required for the lysis of endocytotic vesicles within the target cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acridine Orange
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Adhesins, Bacterial / genetics
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Adhesins, Bacterial / metabolism
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Bacterial Toxins / genetics
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Bacterial Toxins / metabolism
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Biological Transport
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Cell Line, Tumor
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Drug Delivery Systems / methods*
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Endocytosis
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Epithelial Cells / metabolism
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Epithelial Cells / microbiology
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Epithelial Cells / pathology
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Fluorescent Dyes
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Gastric Mucosa / metabolism
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Gastric Mucosa / microbiology
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Gastric Mucosa / pathology
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Gene Expression
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / metabolism
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Hemolysin Proteins / genetics
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Hemolysin Proteins / metabolism
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Humans
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Indoles
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Microscopy, Fluorescence
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Plasmids / chemistry
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Plasmids / metabolism*
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RNA Interference*
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RNA, Small Interfering / chemistry*
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RNA, Small Interfering / genetics
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RNA, Small Interfering / metabolism
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Transport Vesicles / metabolism*
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Transport Vesicles / microbiology
Substances
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Adhesins, Bacterial
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Bacterial Toxins
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Fluorescent Dyes
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Heat-Shock Proteins
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Hemolysin Proteins
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Indoles
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RNA, Small Interfering
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invasin, Yersinia
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DAPI
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Acridine Orange
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hlyA protein, Listeria monocytogenes