Antioxidant Activity Evaluation Involving Hemoglobin-Related Free Radical Reactivity

Methods Mol Biol. 2015;1208:247-55. doi: 10.1007/978-1-4939-1441-8_18.


Two methods for the measurement of antioxidant capacity are described: one based on a chronometric variation of a hemoglobin ascorbate peroxidase assay and the other based on electron paramagnetic resonance (EPR) spectra collected upon alkaline treatment of ethanolic samples. The involved chemical mechanisms are discussed, alongside the most important benefits and shortcomings; the assays offer new qualitative and quantitative information on samples of biological as well as synthetic origin.

MeSH terms

  • Animals
  • Antioxidants / metabolism*
  • Ascorbate Peroxidases / metabolism
  • Ascorbic Acid / metabolism
  • Cattle
  • Electron Spin Resonance Spectroscopy / methods*
  • Free Radicals / metabolism*
  • Hemoglobins / metabolism*
  • Oxidation-Reduction
  • Time Factors


  • Antioxidants
  • Free Radicals
  • Hemoglobins
  • Ascorbate Peroxidases
  • Ascorbic Acid