Quantum dot-based multiphoton fluorescent pipettes for targeted neuronal electrophysiology

Nat Methods. 2014 Dec;11(12):1237-1241. doi: 10.1038/nmeth.3146. Epub 2014 Oct 19.

Abstract

Targeting visually identified neurons for electrophysiological recording is a fundamental neuroscience technique; however, its potential is hampered by poor visualization of pipette tips in deep brain tissue. We describe quantum dot-coated glass pipettes that provide strong two-photon contrast at deeper penetration depths than those achievable with current methods. We demonstrated the pipettes' utility in targeted patch-clamp recording experiments and single-cell electroporation of identified rat and mouse neurons in vitro and in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / physiology*
  • Electrophysiology / instrumentation
  • Electrophysiology / methods*
  • Fluorescent Dyes
  • Luminescent Proteins / metabolism*
  • Mice
  • Microscopy, Fluorescence / instrumentation
  • Microscopy, Fluorescence / methods*
  • Neurons / cytology
  • Neurons / physiology*
  • Optics and Photonics / instrumentation*
  • Optics and Photonics / methods
  • Patch-Clamp Techniques / instrumentation
  • Patch-Clamp Techniques / methods*
  • Quantum Dots*
  • Rats

Substances

  • Fluorescent Dyes
  • Luminescent Proteins