Bialaphos resistance as a dominant selectable marker in Neurospora crassa

Curr Genet. 1989 Dec;16(5-6):369-72. doi: 10.1007/BF00340716.

Abstract

Conidia of Neurospora crassa are sensitive to the herbicide bialaphos at concentrations of 160 mg/l in Westergaard's or Fries' minimal media. Plasmid pJA4 was constructed by inserting a truncated bar gene from Streptomyces hygroscopicus fused to the his-3 promoter from N. crassa into pUC19. The bar gene in plasmid pJA4 confers resistance to bialaphos when transformants are selected on a medium containing bialaphos. The bar gene can be used as an additional dominant selectable marker for transformation of fungi.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Blotting, Southern
  • Drug Resistance, Microbial / genetics
  • Genes, Bacterial
  • Genes, Fungal
  • Genetic Markers
  • Neurospora / genetics*
  • Neurospora crassa / drug effects
  • Neurospora crassa / genetics*
  • Organophosphorus Compounds / pharmacology*
  • Plasmids
  • Promoter Regions, Genetic
  • Restriction Mapping
  • Streptomyces / genetics
  • Transformation, Genetic*

Substances

  • Anti-Bacterial Agents
  • Genetic Markers
  • Organophosphorus Compounds
  • bialaphos