The major dopamine D2 receptor: molecular analysis of the human D2A subtype

DNA. 1989 Nov;8(9):683-9. doi: 10.1089/dna.1.1989.8.683.


The structural diversity of the human D2 dopamine receptor was examined at the nucleic acid level. Sequence analysis of receptor cDNA clones isolated from human brain and pituitary libraries and polymerase chain reaction (PCR) analysis of rat brain RNA and human genomic DNA demonstrate the presence of a predominant D2 subtype, D2A. The D2A subtype differs from the D2B subtype, previously described in rat brain RNA, in that an additional 29 amino acids are present in the putative third cytoplasmic domain, a region thought to be important for coupling to different G-proteins. The demonstration of intron sequences flanking the DNA encoding the 29-amino-acid insertion suggests that the generation of two distinct D2 dopamine receptor subtypes may arise from alternative splicing of a common genomic sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • Cloning, Molecular
  • DNA
  • Humans
  • Introns / genetics
  • Molecular Sequence Data
  • Pituitary Gland / metabolism
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Rats
  • Receptors, Dopamine / classification
  • Receptors, Dopamine / genetics*
  • Receptors, Dopamine D2
  • Sequence Homology, Nucleic Acid


  • RNA, Messenger
  • Receptors, Dopamine
  • Receptors, Dopamine D2
  • DNA

Associated data

  • GENBANK/M30625