Combination of an unbiased amplification method and a resequencing microarray for detecting and genotyping equine arteritis virus

J Clin Microbiol. 2015 Jan;53(1):287-91. doi: 10.1128/JCM.01935-14. Epub 2014 Oct 22.

Abstract

This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arterivirus Infections / diagnosis
  • Arterivirus Infections / veterinary
  • Arterivirus Infections / virology*
  • Equartevirus* / classification
  • Equartevirus* / genetics
  • Genotyping Techniques / methods*
  • Horse Diseases / diagnosis
  • Horse Diseases / virology*
  • Horses
  • Oligonucleotide Array Sequence Analysis / methods*
  • Phylogeny
  • Virology / methods*