Spread of Streptococcus pneumoniae serotype 8-ST63 multidrug-resistant recombinant Clone, Spain

Emerg Infect Dis. 2014 Nov;20(11):1848-56. doi: 10.3201/eid2011.131215.

Abstract

Since 2004, a total of 131 isolates of Streptococcus pneumoniae multidrug-resistant invasive serotype 8 have been detected in Spain. These isolates showed resistance to erythromycin, clindamycin, tetracycline, and ciprofloxacin. All isolates were obtained from adult patients and shared a common genotype (sequence type [ST]63; penicillin-binding protein 1a [pbp1a], pbp2b, and pbp2x gene profiles; ermB and tetM genes; and a ParC-S79F change). Sixty-eight isolates that required a ciprofloxacin MIC ≥16 μg/mL had additional gyrA gene changes. Serotype 8-ST63 pbp2x sequences were identical with those of antimicrobial drug-susceptible serotype 8-ST53 isolates. Serotype 8-ST63 pbp2b sequences were identical with those of the multidrug-resistant Sweden 15A-ST63 clone. Recombination between the capsular locus and flanking regions of an ST53 isolate (donor) and an ST63 pneumococcus (recipient) generated the novel 15A-ST63 clone. One recombination point was upstream of pbp2x and another was within pbp1a. A serotype 8-ST63 clone was identified as a cause of invasive disease in Spain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Amino Acid Sequence
  • Anti-Bacterial Agents / pharmacology
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Base Sequence
  • Child
  • Codon
  • Drug Resistance, Multiple, Bacterial* / genetics
  • Gene Order
  • Genome, Bacterial
  • Genotype
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Molecular Typing
  • Phenotype
  • Pneumococcal Infections / epidemiology*
  • Pneumococcal Infections / microbiology*
  • Polymorphism, Genetic
  • Recombination, Genetic*
  • Serogroup
  • Spain / epidemiology
  • Streptococcus pneumoniae / classification*
  • Streptococcus pneumoniae / genetics
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Codon