Combined effect of high hydrostatic pressure and mild heat treatments on pectin methylesterase (PME) inactivation in comminuted orange

J Sci Food Agric. 2015 Sep;95(12):2438-44. doi: 10.1002/jsfa.6969. Epub 2014 Nov 24.


Background: Comminuted orange, a product obtained by grinding the juice and peel and used to formulate beverages, has a high pectin methylesterase (PME) activity; thus the inactivation of this enzyme is necessary to avoid quality losses related to cloud loss. The use of high hydrostatic pressure (HHP) and mild temperature allows inactivation of enzymes with minimal quality changes. This work aimed to evaluate the effect of pressure, mild temperature and time of treatment, including come-up and holding time, on the inactivation of PME in comminuted orange, and to apply kinetic and response surface models (RSM) to predict residual PME activity (A/A0 ).

Results: During come-up time in treatments at 68 °C, the higher the pressure, the lower was the A/A0 obtained. At 550 MPa/68 °C/10 min the lowest residual activity value was obtained (15.6%). A/A0 was well adjusted to the RSM, and a first-order kinetic model was applied to describe the inactivation of PME. In general, the higher the pressure, the lower was the A/A0 reached, as the increasing values of k from 3.5 × 10(-2) to 55.5 × 10(-2) min(-1) indicated. Activation volume (Va ) values ranging from -9.2 to -17.7 cm(3) mol(-1) , and activation energies (Ea ) between 50.0 and 68.2 kJ mol(-1) were calculated.

Conclusion: 550 MPa/68 °C/10 min, 350 MPa/68 °C/10 min and 450 MPa/56 °C/10 min treatments were satisfactory (∼84% inactivation) to inactivate PME. A first-order kinetic model was applied to describe PME inactivation, and the resulting A/A0 adjusted to the RSM. In addition, linearized Arrhenius and Eyring equations were well fitted in order to obtain Ea and Va , respectively.

Keywords: HHP; PME; comminuted orange; inactivation; kinetic parameters.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Beverages*
  • Carboxylic Ester Hydrolases / chemistry*
  • Citrus sinensis*
  • Food Preservation*
  • Hot Temperature
  • Humans
  • Hydrostatic Pressure


  • Carboxylic Ester Hydrolases
  • pectinesterase