Facile and scalable preparation of pure and dense DNA origami solutions

Angew Chem Int Ed Engl. 2014 Nov 17;53(47):12735-40. doi: 10.1002/anie.201405991. Epub 2014 Oct 24.


DNA has become a prime material for assembling complex three-dimensional objects that promise utility in various areas of application. However, achieving user-defined goals with DNA objects has been hampered by the difficulty to prepare them at arbitrary concentrations and in user-defined solution conditions. Here, we describe a method that solves this problem. The method is based on poly(ethylene glycol)-induced depletion of species with high molecular weight. We demonstrate that our method is applicable to a wide spectrum of DNA shapes and that it achieves excellent recovery yields of target objects up to 97 %, while providing efficient separation from non-integrated DNA strands. DNA objects may be prepared at concentrations up to the limit of solubility, including the possibility for bringing DNA objects into a solid phase. Due to the fidelity and simplicity of our method we anticipate that it will help to catalyze the development of new types of applications that use self-assembled DNA objects.

Keywords: DNA nanotechnology; DNA origami; molecular crowding; poly(ethylene glycol); self-assembly.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / chemical synthesis*
  • DNA / chemistry*
  • Electrophoresis, Agar Gel
  • Molecular Weight
  • Polyethylene Glycols / chemistry
  • Solutions


  • Solutions
  • Polyethylene Glycols
  • DNA