Abstract
We created a novel chimeric amine dehydrogenase (AmDH) via domain shuffling of two parent AmDHs ('L- and F-AmDH'), which in turn had been generated from leucine and phenylalanine DH, respectively. Unlike the parent proteins, the chimeric AmDH ('cFL-AmDH') catalyzes the amination of acetophenone to (R)-methylbenzylamine and adamantylmethylketone to adamantylethylamine.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amination
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Amines / chemistry
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Amino Acid Oxidoreductases / chemistry*
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Amino Acid Oxidoreductases / genetics
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Ketones / chemistry
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Leucine Dehydrogenase / chemistry*
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Leucine Dehydrogenase / genetics
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Recombinant Fusion Proteins / chemistry*
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Substrate Specificity
Substances
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Amines
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Ketones
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Recombinant Fusion Proteins
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Amino Acid Oxidoreductases
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Leucine Dehydrogenase
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phenylalanine oxidase