A real-time PCR approach based on SPF10 primers and the INNO-LiPA HPV genotyping extra assay for the detection and typing of human papillomavirus

Methods Mol Biol. 2015:1249:27-35. doi: 10.1007/978-1-4939-2013-6_2.

Abstract

A highly sensitive SPF10 real-time PCR was developed to achieve simultaneous amplification and detection of the human papillomavirus (HPV) target. That way, LiPA analysis of the HPV-negative samples can be avoided, reducing workload and cost. Here, we describe in detail a SYBR Green I-based real-time PCR assay based on SPF10 primers using the LightCycler(®) 480 system to generate and detect HPV amplicons, which are compatible with the LiPA assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Primers / metabolism*
  • Genotype
  • Genotyping Techniques / methods*
  • Humans
  • Molecular Typing / methods*
  • Nucleic Acid Denaturation / genetics
  • Papillomaviridae / classification*
  • Papillomaviridae / genetics*
  • Papillomaviridae / isolation & purification
  • Real-Time Polymerase Chain Reaction / methods*

Substances

  • DNA Primers