Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 1 (7), 707-14

Functional Architecture of the Light-Responsive Chalcone Synthase Promoter From Parsley

Affiliations

Functional Architecture of the Light-Responsive Chalcone Synthase Promoter From Parsley

P Schulze-Lefert et al. Plant Cell.

Abstract

We have combined in vivo genomic footprinting and light-induced transient expression of chalcone synthase promoter derivatives in parsley protoplasts to identify cis sequences regulating light activation. The parsley chalcone synthase promoter contains two cis "units" that are light-responsive. Each unit is composed of short DNA stretches of approximately 50 base pairs, and each contains two in vivo footprints. One of the footprints in each unit covers a sequence that is highly conserved among other light- and stress-regulated plant genes. The other footprinted sequences in each unit are not related to each other. The TATA distal light-responsive unit is inherently weak but can compensate partially for the loss of the stronger TATA proximal unit. Levels of light-induced expression from either can be influenced by the presence of a region of approximately 100 base pairs located upstream of the TATA distal light-responsive unit. Combination of the light-responsive units and upstream region generates a synergistic response to light. We speculate that functional compensation generated by nonidentical, but sequence-related, cis units foreshadows combinatorial diversity of cognate trans factors.

Similar articles

See all similar articles

Cited by 52 PubMed Central articles

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1988 Jul;85(13):4662-6 - PubMed
    1. EMBO J. 1987 Sep;6(9):2551-6 - PubMed
    1. Science. 1986 Aug 29;233(4767):977-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7089-93 - PubMed
    1. Cell. 1986 Sep 12;46(6):795-805 - PubMed

Publication types

LinkOut - more resources

Feedback