Membrane trafficking. The specificity of vesicle traffic to the Golgi is encoded in the golgin coiled-coil proteins

Science. 2014 Oct 31;346(6209):1256898. doi: 10.1126/science.1256898.


The Golgi apparatus is a multicompartment central sorting station at the intersection of secretory and endocytic vesicular traffic. The mechanisms that permit cargo-loaded transport vesicles from different origins to selectively access different Golgi compartments are incompletely understood. We developed a rerouting and capture assay to investigate systematically the vesicle-tethering activities of 10 widely conserved golgin coiled-coil proteins. We find that subsets of golgins with distinct localizations on the Golgi surface have capture activities toward vesicles of different origins. These findings demonstrate that golgins act as tethers in vivo, and hence the specificity we find to be encoded in this tethering is likely to make a major contribution to the organization of membrane traffic at the Golgi apparatus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantigens / genetics
  • Autoantigens / metabolism
  • Cytoskeletal Proteins
  • Endoplasmic Reticulum / metabolism
  • Endosomes / metabolism*
  • Golgi Apparatus / metabolism*
  • Golgi Matrix Proteins
  • HeLa Cells
  • Humans
  • Membrane Proteins / metabolism
  • Membrane Transport Proteins / metabolism
  • Microscopy, Electron
  • Mitochondria / metabolism*
  • Mitochondria / ultrastructure
  • Nuclear Proteins / metabolism
  • Protein Transport


  • Autoantigens
  • Cytoskeletal Proteins
  • GCC1 protein, human
  • GOLGA4 protein, human
  • Golgi Matrix Proteins
  • Golgi complex autoantigen, 97-kDa
  • Golgin subfamily A member 2
  • Membrane Proteins
  • Membrane Transport Proteins
  • Nuclear Proteins
  • TRIP11 protein, human