TFClass: a classification of human transcription factors and their rodent orthologs

Nucleic Acids Res. 2015 Jan;43(Database issue):D97-102. doi: 10.1093/nar/gku1064. Epub 2014 Oct 31.


TFClass aims at classifying eukaryotic transcription factors (TFs) according to their DNA-binding domains (DBDs). For this, a classification schema comprising four generic levels (superclass, class, family and subfamily) was defined that could accommodate all known DNA-binding human TFs. They were assigned to their (sub-)families as instances at two different levels, the corresponding TF genes and individual gene products (protein isoforms). In the present version, all mouse and rat orthologs have been linked to the human TFs, and the mouse orthologs have been arranged in an independent ontology. Many TFs were assigned with typical DNA-binding patterns and positional weight matrices derived from high-throughput in-vitro binding studies. Predicted TF binding sites from human gene upstream sequences are now also attached to each human TF whenever a PWM was available for this factor or one of his paralogs. TFClass is freely available at through a web interface and for download in OBO format.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • DNA / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / metabolism
  • Databases, Protein*
  • Humans
  • Internet
  • Mice
  • Protein Structure, Tertiary
  • Rats
  • Transcription Factors / chemistry
  • Transcription Factors / classification*
  • Transcription Factors / metabolism


  • DNA-Binding Proteins
  • Transcription Factors
  • DNA