Combined use of Mycobacterium tuberculosis-specific CD4 and CD8 T-cell responses is a powerful diagnostic tool of active tuberculosis

Virginie Rozot; Amelio Patrizia; Selena Vigano; Jesica Mazza-Stalder; Elita Idrizi; Cheryl L Day; Matthieu Perreau; Catherine Lazor-Blanchet; Khalid Ohmiti; Delia Goletti; Pierre-Alexandre Bart; Willem Hanekom; Thomas J Scriba; Laurent Nicod; Giuseppe Pantaleo; Alexandre Harari

doi:10.1093/cid/ciu795

Combined use of Mycobacterium tuberculosis-specific CD4 and CD8 T-cell responses is a powerful diagnostic tool of active tuberculosis

Clin Infect Dis. 2015 Feb 1;60(3):432-7. doi: 10.1093/cid/ciu795. Epub 2014 Oct 31.

Authors

Virginie Rozot¹, Amelio Patrizia², Selena Vigano², Jesica Mazza-Stalder³, Elita Idrizi², Cheryl L Day⁴, Matthieu Perreau², Catherine Lazor-Blanchet⁵, Khalid Ohmiti², Delia Goletti⁶, Pierre-Alexandre Bart², Willem Hanekom⁴, Thomas J Scriba⁴, Laurent Nicod³, Giuseppe Pantaleo⁷, Alexandre Harari⁷

Affiliations

¹ Division of Immunology and Allergy, Lausanne University Hospital, Switzerland South African Tuberculosis Vaccine Initiative, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, South Africa.
² Division of Immunology and Allergy, Lausanne University Hospital, Switzerland.
³ Division of Pneumology.
⁴ South African Tuberculosis Vaccine Initiative, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, South Africa.
⁵ Occupational Medicine, Centre Hospitalier Universitaire Vaudois, University of Lausanne, Switzerland.
⁶ National Institute for Infectious Diseases, Rome, Italy.
⁷ Division of Immunology and Allergy, Lausanne University Hospital, Switzerland Swiss Vaccine Research Institute, Lausanne, Switzerland.

Abstract

Immune-based assays are promising tools to help to formulate diagnosis of active tuberculosis. A multiparameter flow cytometry assay assessing T-cell responses specific to Mycobacterium tuberculosis and the combination of both CD4 and CD8 T-cell responses accurately discriminated between active tuberculosis and latent infection.

Keywords: CD8 T cells; active tuberculosis disease; diagnosis; functional profile; latent Mtb infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Antigens, Bacterial / immunology*
CD4-Positive T-Lymphocytes / immunology*
CD8-Positive T-Lymphocytes / immunology*
Child
Female
Flow Cytometry
Humans
Male
Middle Aged
Mycobacterium tuberculosis / immunology*
Tuberculosis / diagnosis*
Tuberculosis / immunology
Young Adult

Substances

Antigens, Bacterial

Abstract

Publication types

MeSH terms

Substances

Grants and funding