DNA Synthesis in Mouse Brown Adipose Tissue Is Under Beta-Adrenergic Control

Exp Cell Res. 1989 Feb;180(2):574-9. doi: 10.1016/0014-4827(89)90086-4.

Abstract

The rate of DNA synthesis in mouse brown adipose tissue was followed with injections of [3H]thymidine. Cold exposure led to a large increase in the rate of [3H]thymidine incorporation, reaching a maximum after 8 days, whereafter the activity abruptly ceased. A series of norepinephrine injections was in itself able to increase [3H]thymidine incorporation. When norepinephrine was injected in combination with the alpha-adrenergic antagonist phentolamine or with the beta-adrenergic antagonist propranolol, the stimulation was fully blocked by propranolol. It is suggested that stimulation of DNA synthesis in brown adipose tissue is a beta-adrenergically mediated process and that the tissue is an interesting model for studies of physiological control of DNA synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue, Brown / metabolism*
  • Adipose Tissue, Brown / physiology
  • Animals
  • DNA Replication / drug effects*
  • Female
  • Mice
  • Norepinephrine / administration & dosage
  • Norepinephrine / pharmacology
  • Phentolamine / administration & dosage
  • Propranolol / administration & dosage
  • Receptors, Adrenergic, alpha / drug effects
  • Receptors, Adrenergic, alpha / physiology
  • Receptors, Adrenergic, beta / drug effects
  • Receptors, Adrenergic, beta / physiology*
  • Thymidine / administration & dosage
  • Thymidine / physiology

Substances

  • Receptors, Adrenergic, alpha
  • Receptors, Adrenergic, beta
  • Propranolol
  • Thymidine
  • Norepinephrine
  • Phentolamine