The effects of hyaluronic acid vaginal gel on the vaginal epithelium of ovariectomized rats

Gynecol Endocrinol. 2015 Mar;31(3):208-13. doi: 10.3109/09513590.2014.975689. Epub 2014 Nov 4.


Objective: Hyaluronic acid is one of the best materials of water retention which can be used in vaginal atrophy. This study is to evaluate the role and mechanism of the hyaluronic acid vaginal gel (Hyalofemme) in the vaginal epithelium of ovariectomized rats.

Materials and methods: Sixty SD rats were randomly divided into control group (Sham ovariectomy, Sham-OVX), tendency group (ovariectomy, OVX), and experiment group (ovariectomy+Hyalofemme, OVX+Hyalofemme). The hyaluronic acid vaginal gel was administered local vaginal therapy to the experiment group with cytologicaly confirmed vaginal atrophy. The doses were adjusted by animal weight according to human dosage. After daily treatment for 14 days, VEGF and P-AKT activations were detected by Western blot in the experiment group.

Results: The hyaluronic acid vaginal gel proved to be very effective in the cytological reversal of vaginal atrophy but did not increase uterine weight. Vaginal microecosystem indicators were negative in the control group and the experiment group. By contrast, the indicators were positive in the tendency group.

Conclusion: Hyaluronic acid vaginal gel is effective in the reversal of vaginal atrophy and is beneficial for improving vaginal microecosystem in the postmenopausal rat model. The hyaluronic acid vaginal gel can also improve the repair capacity of the vaginal epithelium.

Keywords: Hyaluronic acid; ovariectomized rats; postmenopausal rat model; vaginal epithelium; vaginal gel.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intravaginal
  • Animals
  • Atrophy / drug therapy
  • Atrophy / pathology
  • Disease Models, Animal
  • Epithelium / drug effects*
  • Epithelium / pathology
  • Female
  • Hyaluronic Acid / pharmacology*
  • Hyaluronic Acid / therapeutic use
  • Rats
  • Rats, Sprague-Dawley
  • Vagina / drug effects*
  • Vagina / pathology
  • Vaginal Creams, Foams, and Jellies / pharmacology
  • Vaginal Creams, Foams, and Jellies / therapeutic use
  • Vaginal Diseases / drug therapy*
  • Vaginal Diseases / pathology


  • Vaginal Creams, Foams, and Jellies
  • Hyaluronic Acid