Agavins from Agave angustifolia and Agave potatorum affect food intake, body weight gain and satiety-related hormones (GLP-1 and ghrelin) in mice

Food Funct. 2014 Dec;5(12):3311-9. doi: 10.1039/c4fo00561a.


Agavins act as a fermentable dietary fiber and have attracted attention due to their potential for reducing the risk of disease. Therefore, we evaluated the effect of supplementation using 10% agavins with a short-degree of polymerization (SDP) from Agave angustifolia Haw. (AASDP) or Agave potatorum Zucc. (APSDP) along with chicory fructans (RSE) as a reference for 5 weeks, on the energy intake, body weight gain, satiety-related hormones from the gut and blood (GLP-1 and ghrelin), blood glucose and lipids, and short-chain fatty acids (SCFAs) from the gut of ad libitum-fed mice. We evaluated the energy intake daily and weight gain every week. At the end of the experiment, portal vein blood samples as well as intestinal segments and the stomach were collected to measure glucagon-like peptide-1 (GLP-1) and ghrelin using RIA and ELISA kits, respectively. Colon SCFAs were measured using gas chromatography. The energy intake, body weight gain, and triglycerides were lower in the fructan-fed mice than in the STD-fed mice. The AASDP, APSDP, and RSE diets increased the serum levels of GLP-1 (40, 93, and 16%, respectively vs. STD) (P ≤ 0.05), whereas ghrelin was decreased (16, 38, and 42%, respectively) (P ≤ 0.05). Butyric acid increased significantly in the APSDP-fed mice (26.59 mmol g(-1), P ≤ 0.001) compared with that in the AASDP- and RSE-fed mice. We concluded that AASDP and APSDP are able to promote the secretion of the peptides involved in appetite regulation, which might help to control obesity and its associated metabolic disorder.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agave / metabolism*
  • Animals
  • Blood Glucose / metabolism
  • Dietary Fiber / metabolism*
  • Eating*
  • Energy Intake
  • Ghrelin / blood*
  • Glucagon-Like Peptide 1 / blood*
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Obesity / diet therapy*
  • Obesity / metabolism
  • Obesity / physiopathology
  • Triglycerides / blood
  • Weight Gain*


  • Blood Glucose
  • Dietary Fiber
  • Ghrelin
  • Triglycerides
  • Glucagon-Like Peptide 1