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. 2014 Nov;34(11):6327-32.

Effect of Withania Somnifera Root Extract on Spontaneous Estrogen Receptor-Negative Mammary Cancer in MMTV/Neu Mice

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Free PMC article

Effect of Withania Somnifera Root Extract on Spontaneous Estrogen Receptor-Negative Mammary Cancer in MMTV/Neu Mice

Kamel F Khazal et al. Anticancer Res. .
Free PMC article

Abstract

The cancer-preventive activity of an extract of Withania somnifera (WS) roots was examined in female transgenic (MMTV/Neu) mice that received a diet containing the extract (750 mg/kg of diet) for 10 months. Mice in the treated group (n=35) had an average of 1.66 mammary carcinomas, and mice in the control group (n=33) had 2.48, showing a reduction of 33%. The average weights of the carcinomas were 2.36 g for mice in the treated group and 2.63 g for the controls, a difference of 10%. Labeling indices for Ki67 and proliferating cell nuclear antigen marker in mammary carcinomas of the treated group were 35% and 30% lower, respectively, than those of the corresponding control group. Expression of the chemokine was reduced by 50%. These results indicate that the root extract reduced the number of mammary carcinomas that developed and reduced the rate of cell division in the carcinomas.

Keywords: Withania; chemoprevention; mammary cancer; mice.

Figures

Figure 1
Figure 1
Effect of the WS extract on development of mammary carcinomas in transgenic MMTV/Neu mice. Group 1 mice received WS root extract (750 mg/kg diet) for 10 months and Group 2 standard diet only.
Figure 2
Figure 2
Hematoxylin and eosin (H&E), Ki67, and PCNA staining of the mammary glands of control and WS-treated mice (magnification ×20). A: H&E-stained sections of mammary carcinomas in WS-treated and untreated mice. Mammary carcinomas of treated mice showed decreased staining for Ki67(B), PCNA (C) and cytokine CCL2 (D). E: Labeling indices, as determined by Ki67 and PCNA staining, and CCL2 were 42%, 38% and 50% less, respectively, in treated (Trtd) mice than in those of controls (Crtl).

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