The effect of weight reduction on antioxidant enzymes and their association with dietary intake of vitamins A, C and E

Arq Bras Endocrinol Metabol. 2014 Oct;58(7):744-9. doi: 10.1590/0004-2730000003206.


Objective: Our goal was to assess the effects of weight loss on antioxidant enzymes of red blood cells and it's relation with vitamins A, E and C intake in 30 obese women.

Subjects and methods: General information, anthropometric measurements, 3-day food recall, and fasting blood samples were collected from 30 obese women at the beginning of the study and after 3 months intervention. Weight loss was set at about 10% of their weight before the intervention.

Results: Glutathione reductase and catalase activities showed a significant increase (P < 0.01) after weight reduction, but no significant changes were seen in the superoxide dismutase and glutathione peroxidase activities. There was a positive linear correlation between daily vitamin C intake with superoxide dismutase enzyme after intervention (P = 0.004, r = 0.507). There was a negative linear correlation between vitamin E intake and glutathione peroxidase activity before intervention (P = 0.005, r = -0.5). A negative correlation was found between daily vitamin A intake and glutathione reductase enzyme before and after intervention (r = -0.385, r = -0.397, P < 0.05) respectively. No significant correlation was observed between vitamins A, C, E amounts and catalase activity.

Conclusions: Ten percent weight reduction can have a significant role in increasing antioxidant enzymes activities, especially glutathione reductase, and catalase enzymes in obese women. However, it is important to take into consideration a balanced amount of certain nutrients while administering a diet with limited energy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antioxidants / metabolism
  • Ascorbic Acid / administration & dosage*
  • Ascorbic Acid / metabolism
  • Body Weight / physiology
  • Caloric Restriction
  • Catalase / blood
  • Female
  • Glutathione Peroxidase / blood
  • Glutathione Reductase / blood
  • Hemoglobins / analysis
  • Humans
  • Middle Aged
  • Non-Randomized Controlled Trials as Topic / methods
  • Obesity / diet therapy*
  • Oxidoreductases / analysis
  • Oxidoreductases / metabolism*
  • Superoxide Dismutase / blood
  • Vitamin A / administration & dosage*
  • Vitamin A / metabolism
  • Vitamin E / administration & dosage*
  • Vitamin E / metabolism*
  • Weight Loss / drug effects
  • Weight Loss / physiology*
  • Young Adult


  • Antioxidants
  • Hemoglobins
  • Vitamin A
  • Vitamin E
  • Oxidoreductases
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione Reductase
  • Ascorbic Acid